Structure of the Macrobrachium rosenbergii nodavirus: A new genus within the Nodaviridae?
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منابع مشابه
Tracking the virus-like particles of Macrobrachium rosenbergii nodavirus in insect cells
Macrobrachium rosenbergii nodavirus (MrNv) poses a major threat to the prawn industry. Currently, no effective vaccine and treatment are available to prevent the spread of MrNv. Its infection mechanism and localisation in a host cell are also not well characterised. The MrNv capsid protein (MrNvc) produced in Escherichia coli self-assembled into virus-like particles (VLPs) resembling the native...
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A pathogen of giant freshwater prawn, Macrobrachium rosenbergii, was recently recorded in a hatchery in Yogyakarta. The clinical symptom in post-larvae (PL) was a whitish appearance of the muscles in the tail. Histological examination revealed myonecrosis with massive infiltration of myonuclei and hemocytes. RT-PCR products of 850 bp were obtained when using RNA from diseased PL as a template. ...
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15 صفحه اولProduction of monoclonal antibodies specific to Macrobrachium rosenbergii nodavirus using recombinant capsid protein.
The gene encoding the capsid protein of Macrobrachium rosenbergii nodavirus (MrNV) was cloned into pGEX-6P-1 expression vector and then transformed into the Escherichia coli strain BL21. After induction, capsid protein-glutathione-S-transferase (GST-MrNV; 64 kDa) was produced. The recombinant protein was separated using SDS-PAGE, excised from the gel, electro-eluted and then used for immunizati...
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ژورنال
عنوان ژورنال: PLOS Biology
سال: 2018
ISSN: 1545-7885
DOI: 10.1371/journal.pbio.3000038